Abstract

A new study of the effect of a previously unstudied factor on dispersion, and repeatability of the injected sample. The design idea is based on manufacturing two new microcells with different volumes used in flow injection (FI) analysis systems and determining the measurements of the external structure, internal paths, and the measuring chamber with high accuracy. The efficiency of the FI system was tested by measuring the dead volume, and it was found to be zero. All measurements (n=5) were implemented by using the concentration 10.00 mg/L of Toluidine Blue dye solution at the wavelength 625.0 nm, flow cells with the volumes (35.0, 80.0, and 450.0) μL, sample volumes (39.25 and 78.50) μL, and flow rates (2.6, 4.5, and 6.6) mL/min. The results of the study indicate a relationship between flow cell volume and dispersion values. This relationship becomes apparent when other factors, such as sample volume and flow rate, are changed.

Keywords: homemade micro flow cells, dispersion, repeatability, dead volume, toluidine blue

Article language: En