Analytical chemistry council

METHODS AND OBJECTS OF CHEMICAL ANALYSIS

 

An international journal devoted to all aspects of analytical chemistry

ISSN 2413-6166 (Online), ISSN 1991-0290 (Print)

Kiev University
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Development and Validation of a Bioanalytical Method for the Quantification of Clopidogrel in Human Plasma by LC-MS/MS using the Accuracy Profiles

Choukri El Khabbaz†, Aimen El Orche*‡, Mustapha Bouatia§, Amine Cheikhǁ, My El Abbes Faouzi₮, Yahia Cherrahǁ, Ratiba Boussen†, Houda Bouchafra#

* Corresponding authors

† Laboratory of Materials Nanotechnologies and Environment, Faculty of Science, Mohammed V University, Rabat, Morocco;
‡ Team of Analytical and Computational Chemistry, Nanotechnology and Environment, Faculty of Science and Techniques, University of Sultan Moulay, Slimane, Beni Mellal-Morocco;
§ Laboratory of Analytical Chemistry, Faculty of Medicine and Pharmacy, Mohammed V University, Rabat, Morocco;
ǁ Department of Pharmacy, Faculty of Pharmacy, Abulcasis University, Rabat, Morocco;
₮ Laboratory of Pharmacology and Toxicology, Faculty of Medicine and Pharmacy, Mohammed V University, Rabat, Morocco;
# Laboratory of Drug Sciences, Biomedical and Technological Research, Faculty of Medicine and Pharmacy, Hassane II University, Casablanca, Morocco;

*e-mail: aimen.elorche@gmail.com;

Methods Objects Chem. Anal., 2022, 17(3), p. 153-161

https://doi.org/10.17721/moca.2022.153-161

The article is distributed under open access Creative Commons Attribution License CC BY 4.0.

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Abstract

A basic bio-analytical method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed for the determination of clopidogrel in human plasma. Clopidogrel-d3 was used as an internal standard (IS). Protein precipitation with acetonitrile for extraction of clopidogrel and it IS was used. Chromatographic separation of clopidogrel and IS was performed on a Synergi reverse phase C18 column (150 mm × 2 mm, 4 μm) using an isocratic mode. The mobile phase contained a mixture of 0.1% formic acid and acetonitrile (25 : 75) applying a flow rate of 0.4 mL/min. Ionization was performed by an electrospray ionization source (ESI), in positive MRM (Multiple Reaction Monitoring) mode. This method was validated by accuracy profiles approach using tolerance intervals limits. The calibration curve ranged from 0.0156 ng/mL to 8 ng/mL. This method can be employed effectively in bioequivalence and/or pharmacokinetics. In this method we have avoided the use of methanol in all steps of bioanalysis of clopidogrel to avert the back-conversion of clopidogrel metabolite.

Keywords: tandem mass spectrometry, clopidogrel, accuracy profiles

Article language: En


Publisher: Taras Shevchenko National University, Kiev Ukraine

(Analytical chemistry department at Taras Shevchenko National University, 64 Vladimirskaya STR., Kiev, 01601 UKRAINE)
analysis@univ.kiev.ua, www.univ.kiev.ua, +380 (44) 2393444

The journal is indexed in SCOPUS and Web of Science

The journal website: www.moca.net.ua

The articles in this journal are licensed under CC BY 4.0