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METHODS AND OBJECTS OF CHEMICAL ANALYSIS
An international journal devoted to all aspects of analytical chemistry ISSN 2413-6166 (Online), ISSN 1991-0290 (Print) |
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Development and Validation of a Bioanalytical Method for the Quantification of Clopidogrel in Human Plasma by LC-MS/MS using the Accuracy Profiles |
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* Corresponding authors
† Laboratory of Materials Nanotechnologies and Environment, Faculty of Science, Mohammed V University, Rabat, Morocco; *e-mail: aimen.elorche@gmail.com; |
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Methods Objects Chem. Anal., 2022, 17(3), p. 153-161 https://doi.org/10.17721/moca.2022.153-161 The article is distributed under open access Creative Commons Attribution License CC BY 4.0. |
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Abstract
A basic bio-analytical method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed for the determination of clopidogrel in human plasma. Clopidogrel-d3 was used as an internal standard (IS). Protein precipitation with acetonitrile for extraction of clopidogrel and it IS was used. Chromatographic separation of clopidogrel and IS was performed on a Synergi reverse phase C18 column (150 mm × 2 mm, 4 μm) using an isocratic mode. The mobile phase contained a mixture of 0.1% formic acid and acetonitrile (25 : 75) applying a flow rate of 0.4 mL/min. Ionization was performed by an electrospray ionization source (ESI), in positive MRM (Multiple Reaction Monitoring) mode. This method was validated by accuracy profiles approach using tolerance intervals limits. The calibration curve ranged from 0.0156 ng/mL to 8 ng/mL. This method can be employed effectively in bioequivalence and/or pharmacokinetics. In this method we have avoided the use of methanol in all steps of bioanalysis of clopidogrel to avert the back-conversion of clopidogrel metabolite.
Keywords: tandem mass spectrometry, clopidogrel, accuracy profiles
Article language: En
Publisher: Taras Shevchenko National University, Kiev Ukraine
(Analytical chemistry department at Taras Shevchenko
National University, 64 Vladimirskaya STR., Kiev, 01601 UKRAINE)
analysis@univ.kiev.ua,
www.univ.kiev.ua,
+380 (44) 2393444
The journal is indexed in SCOPUS and Web of Science
The journal website: www.moca.net.ua
The articles in this journal are licensed under
CC BY 4.0

