Biologically Active Aldehydes in Extracts of Lactarius Pergamenus (Fr.) Fr Fresh Fruiting Bodies V.O. Antonyuk*†‡, L.V. Panchak†, M.V. Tsivinska§#, R.S. Stoika‡§
	* Corresponding authors †Danylo Halytsky Lviv National Medical University, Pekarska Str., 69, Lviv, 79010, Ukraine; ‡Institute of Cell Biology, National Academy of Sciences of Ukraine, Drahomanov Str., 14/16, Lviv, 79005, Ukraine; §Ivan Franko National University of Lviv, Universitetska Str.,1, 79000, Ukraine; #Scientific-Research Center of Criminal Expertise, Ministry of Internal Affairs of Ukraine in Lviv region; Koniushynna Str., 24, Lviv, Ukraine; *e-mail: antonyukvo@gmail.com
	Methods Objects Chem. Anal., 2020, 15(3), p. 125-131 https://doi.org/10.17721/moca.2020.125-131
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Abstract

The biologically active aldehydes in extracts of fungi of the genus Lactarius were identified. It’s established that these substances are unstable, they are found in fresh and frozen fungi, but are absent in dried mushrooms and interact with 1,4-phenylenediamine to form a colored compound. Methylene chloride is the best extragent for these substances. TLC on silufol plates showed that there were several substances in Lactarius pergamenus fruiting bodies and they had varying degrees of stability. For selection of these substances, methylene chloride extract was separated on a column of silica gel. Fraction, which gave the most expressive reaction with 1,4-phenylenediamine on thin-layer chromatograms were analyzed by GC-MS both in the absence and in the presence of 1,4-phenylenediamine. As a result, it was found that 1,4-phenylenediamine or other aromatic amines interacted with highly active aldehydes, that were present in fruiting bodies. Among them 2,2-dimethylocta- 3,4-dienal was the most stable and was present in the biggest quantity. This substance very rarely occurs in the vegetable kingdom and in fungi extracts of Lactarius genus wasn’t previously described. Its possible function in fungi is prevention of damage by parasites and eating by animals.

Keywords: Lactarius pergamenus (Fr.) Fr, aldehydes, identification, 1,4-phenylenediamine, gas chromatographymass spectrometry, 2.2 dimethylocta-3,4-dienal

Article language: En