ASSESSMENT OF THE RELATIVE RETENTION TIMES VARIABILITY USING CHROMATOGRAPHIC COLUMNS WITH GRAFTED C18 GROUPS I.V. KUDRIS†,§, A.Yu. KULIKOV‡* * Corresponding author † The State Expert Center of the Ministry of Health of Ukraine, Ushinskogo Str., 40, Kiev, Ukraine, 03151 ‡ Laboratory of Pharmacopoeial Analysis, Scientific and Expert Pharmacopoeial Centre, Astronomicheskaya street 33, 61085, Kharkov § V.N. Karazin Kharkiv National University, Svobody Sq. 4, 61022, Kharkiv, Ukraine Email: kulikov@phukr.kharkov.ua Methods Objects Chem. Anal., Volume 9, No 1, 2014 p. 12-18 https://doi.org/10.17721/moca.2014.12-18
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Abstract

One of the ways is used for peaks identification in the chromatograms is relative retention time (RRT). Hoverer, in practice, the val-ues of the relative retention times, which were received, often do not coincide with those prescribed in the literature, and identification, based on the RRT, may lead to incorrect peak assignments. The main contribution on the different retention of compounds influences different chromatographic columns using. In this study the chromatographic behavior of three model mixes was performed by using 11 different chromatographic columns with octadecilsilica sorbent (C18). Variability of the components relative reten-tion times on the different columns was carried out. It is founded that high variability of the relative reten-tion time values on different chromatographic columns do not allow to peaks identification based on RRT.

Keywords: relative retention time, variability, HPLC, octadecylsilica sorbent