METHODS AND OBJECTS OF CHEMICAL ANALYSIS ISSN title: "Metody i obʺekty himičeskogo analiza" An international journal devoted to all aspects of Analytical chemistry ISSN 2413-6166 (Online), ISSN 1991-0290 (Print)
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 AMPEROMETRIC BIOSENSOR FOR THE D-SORBITOL BASED ON MODIFIED WITH BIOCOMPOSITE FILM GLASSY CARBON ELECTRODE.

E.A. MAZURENKO, O.V. SHEVCHENKO, O.Yu. TANANAIKO, M. ETIENNE, A. WALCARIUS

The method of electrophoretic deposition is used here for the obtaining a carbon nanotubes layer on the surface of glassy carbon electrode. The electrode modified with carbon nanotubes demonstrated significant shift of the oxidation potential of co-factor NADH (ΔЕ = 0.25 V) and enhanced stability of the electrochemical response compare to non-modified electrode. The D-sorbitol dehydrogenase enzyme was immobilized into the silica film on the surface of carbon nanotubes-modified electrode. The developed biocomposite electrode was applied for the D-sorbitol determination in foodstuffs and cosmetics with the limit of detection 0.1 mM. The prospects of application of developed electrode as a sensitive element of amperometric biosensor are discussed.