METHODS AND OBJECTS OF CHEMICAL ANALYSIS ISSN title: "Metody i obʺekty himičeskogo analiza" An international journal devoted to all aspects of Analytical chemistry ISSN 2413-6166 (Online), ISSN 1991-0290 (Print)
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The aim of the present study was to evaluate selenium concentration in hair samples. We studied 48 children, aged from 1 to 8 years, living in Kharkov region. The analysis of Se was performed by using micellar liquid chromatographic technique after hair microwave digestion and precolumn derivatization with 2,3,diaminonaphthalene. The method was developed using Hypersil ODS column, UV detection at 378 nm and 10% (v/v) 1,butanol in 0.05 M sodium dodecyl sulfate as the mobile phase, with the retention times below 10 min. Different validation parameters were evaluated. Linearity was established in the range 10,531 ng/mL of selenium (IV) content. The found limits of detection and quantitation are 1.6 ng/mL and 4.7 ng/mL (equivalent to 40 μg/kg of undried hair), respectively. This method can be used for the selenium monitoring and clinical investigations.

Keywords: Micellar liquid chromatography; hair; selenium (IV); 2,3,diaminonaphthalene; naphthylpiazoselenol; sodium dodecyl sulfate; microwave digestion