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METHODS AND OBJECTS OF CHEMICAL ANALYSIS
An international journal devoted to all aspects of analytical chemistry ISSN 2413-6166 (Online), ISSN 1991-0290 (Print) |
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Optimization of ICP-MS Analysis for Studying Matrix Effects in Biological Fluids |
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* Corresponding authors
† Institute of Physics of the Ministry of Science and Education of Republic of Azerbaijan, H. Javid Avenue, 131,
Baku, AZ-1073, Azerbaijan; |
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Methods Objects Chem. Anal., 2025, 20(1), p. 39-45 https://doi.org/10.17721/moca.2025.39-45 The article is distributed under open access Creative Commons Attribution License CC BY 4.0. |
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Abstract
A spectrophotometric method was developed for the precise estimation of clonidine hydrochloride (C-HCl) in pure form and pharmaceutical formulations. This method utilizes an ion-pair interaction with 1,2-naphthoquinone-4-sulphonic acid (NQS) in a basic medium (pH 13) at 55°C, producing a peach-colored solution with maximum absorbance at 475 nm. The method demonstrated linearity over 25-225 μg/mL with a molar absorptivity of 2162 L·mol-¹·cm-¹ (R² = 0.9986) and LOD and LOQ values of 6.28 and 19.02 μg/mL, respectively. The average recovery ranged from 96.4% to 101.4%. Kinetic studies indicated pseudo-firstorder kinetics, while thermodynamic analysis using Eyring and Arrhenius equations revealed the reaction to be endothermic and non-spontaneous. The method proved accurate and suitable for C-HCl analysis in pharmaceutical samples.
Keywords: clonidine hydrochloride, NQS, ion-pair interaction, kinetic spectrophotometry
Article language: En
Publisher: Taras Shevchenko National University, Kiev Ukraine
(Analytical chemistry department at Taras Shevchenko
National University, 64 Vladimirskaya STR., Kiev, 01601 UKRAINE)
analysis@univ.kiev.ua,
www.univ.kiev.ua,
+380 (44) 2393444
The journal is indexed in SCOPUS and Web of Science
The journal website: www.moca.net.ua
The articles in this journal are licensed under
CC BY 4.0
