Abstract

A simple, economical and sensitive indirect spectrophotometric method for the determination of furosemide in its pure form and in pharmaceutical preparations is described. The method is based on the oxidation of furosemide by permanganate in acidic medium, then the reaction of the remaining potassium permanganate to oxidize the dye Eriochrome Black-T at a wavelength of 535 nm. The method follows Beer's law within the range (0.25-10) μg mL-1 and the molar absorbance is 1.5×10⁴ L mol^-1 cm^-1. The value of the determination coefficient was 0.9984, the recovery rate was 100.53 % and the relative error value was 1.12 %, while the value of the limit of detection (LOD) and limit of quantification was (LOQ) 0.118 and 0.395 μg mL^-1 respectively. It was found that the method does not suffer from interactions, and the method can be applied to pharmaceutical preparations (ampoules and tablets).

Keywords: spectrophotometry, furosemide, oxidation, eriochrome black-T dye

Article language: En