Analytical chemistry council

METHODS AND OBJECTS OF CHEMICAL ANALYSIS

ISSN title: "Metody i obʺekty himičeskogo analiza"

An international journal devoted to all aspects of Analytical chemistry

ISSN 2413-6166 (Online), ISSN 1991-0290 (Print)

Kiev University
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Enhanced Electrochemical Determination of Riboflavin in Biological and Pharmaceutical samples at Poly (Arginine) Modified Carbon Paste Electrode

Girish Tigari, J.G. Manjunatha*, D.K. Ravishankar, G. Siddaraju

* Corresponding authors

Department of chemistry FMKMC College, Madikeri, Mangalore university constituent college, Karnataka, India;
*e-mail: manju1853@gmail.com

Methods Objects Chem. Anal., 2019, 14(4), p. 216-223

https://doi.org/10.17721/moca.2019.216-223

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Abstract

An electrogenerated Polyarginine modified carbon paste electrode (PAMCPE) was fabricated through a simple electropolymerization procedure. The devised electrode was characterized by cyclic voltammetry (CV) and Field Emission Scanning Electron Microscopy (FESEM). This electrode was utilized for electrocatalytic estimation of Riboflavin (RF) and its instantaneous resolution with ascorbic acid (AA) and folic acid (FA) in phosphate buffer solution (PBS) of pH 6.0 by differential pulse voltammetry (DPV). It was observed to be a very responsive electrode for the electrochemical detection and quantification of RF. It was revealed that PAMCPE generates higher current response towards RF contrast to the bare carbon paste electrode (BCPE). Under optimized condition, the RF oxidation current values were linearly reliant on the RF concentration increment with a limit of detection (LOD) of 9.310-8 M using DPV. The stable PAMCPE was effectively applied for estimation of RF in B-complex pill and complex human blood serum samples.

Keywords: electrochemical sensor, poly(arginine), carbon paste electrode, vitamin B2, human blood serum, B-complex capsule

Article language: En


Publisher:Taras Shevchenko National University, Kiev Ukraine

(Analytical chemistry department at Taras Shevchenko National University, 64 Vladimirskaya STR., Kiev, 01601 UKRAINE)
analysis@univ.kiev.ua, www.achem.univ.kiev.ua, +380-44-2393266

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The journal website: www.moca.net.ua

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