Analytical chemistry council

METHODS AND OBJECTS OF CHEMICAL ANALYSIS

(Metody i Ob''ekty Himicheskogo Analiza)

An international journal devoted to all aspects of Analytical chemistry

ISSN 2413-6166 (Online), ISSN 1991-0290 (Print)

Kiev University
 

Home Current issueArchiveCall for papars Contact us

cover

Assessment of the Conformity of the Methods for Aflatoxin B1 and Deoxynivalenol Determination in Grain and Feeds by Method of High-Performance Liquid Chromatography

O.M. Yakubchak, O.A. Laposha, S.V. Midyk*, T.V. Taran, I.V. Zabarna

* Corresponding authors
National University of Life and Environmental Sciences of Ukraine, Potechina st., 16, Kyiv, 03041;
The State Agrarian and Engineering University in Podilia, Shevchenko str, 13, Kamianets-Podilskyi, Ukraine, 32300;
*e-mail: svit.mid@gmail.com

Methods Objects Chem. Anal., 2018, 13(3), p. 121-130

https://doi.org/10.17721/moca.2018.121-130 

Buy Article:

2 USD plus tax

DOI No.

preview the article

Abstract

The suitability of methods for determination of residual amounts of aflatoxin B1 and deoxynivalenol in grain, products and feeds from grain by the method of high-performance liquid chromatography with validation criteria was evaluated: linearity, detection limit, specificity, intralaboratory reproducibility, correctness (return). It was concluded that the methods for determining in cereal cultures of aflatoxin B1 and deoxynivalenol content with purification on immunoaffinity columns by the method of high-performance liquid chromatography are suitable for the study of grain, products and feeds from grain and can be used by laboratories for conducting similar studies. Adapted methods of mycotoxins determination are highly sensitive and meet European requirements according to their parameters. The recovery percentage is 85.8 3.65 % for all the concentrations of aflatoxin B1 analyzed. It corresponds to the minimum allowable value according to Commission Decision 2002/657 / EC, with the coefficient of variation (CV,%) being 2.13 0.67 and is in accordance with Commission Decision 2002/657 / EC. The recovery rate is 89.2 5.3% for all analyzed concentrations of deoxynivalenol, which corresponds to the minimum allowable value according to Commission Decision 2002/657 / EC, the coefficient of variation (CV, %) is 9.4 1.65 and is suitable

Keywords: mycotoxins, aflatoxin B1, deoxynivalenol, grain, feed, high-performance liquid chromatography

Article language: Ukr


Publisher:Taras Shevchenko National University, Kiev Ukraine

(Analytical chemistry department at Taras Shevchenko National University, 64 Vladimirskaya STR., Kiev, 01601 UKRAINE)
analysis@univ.kiev.ua, www.achem.univ.kiev.ua, +380-44-2393345

Papers published in Methods and Objects of Chemical Analysis are considered as publications suitable for PhD thesis

The journal website: www.moca.net.ua