Analytical chemistry council


(Metody i Ob''ekty Himicheskogo Analiza)

An international journal devoted to all aspects of Analytical chemistry

ISSN 2413-6166 (Online), ISSN 1991-0290 (Print)

Kiev University

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Determination of N-Acetylcysteine in Tablets by Means of Chemiluminescence Inhibition Method

N.Yu. Bondarenko*, M.Ye. Blazheyevskiy

* Corresponding author

National University of Pharmacy, 4 Valentynivska Street, Kharkiv, Ukraine, 61168;

Methods Objects Chem. Anal., 2018, 13(3), p. 110-114

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The technique for quantitative determination of N-acetylcysteine in pharmaceutical preparations using a new chemiluminescence inhibitor – N-acetylcysteine in the system H2L (luminol)–Н2О2–Нemoglobin was developed. The objects of research were tablets containing N-acetylcysteine. The method of N-acetylcysteine quantitative determination in pharmaceuticals based on the inhibition of chemiluminescence in the system H2L–H2O2– Нemoglobin was developed. The calibration curve was linear over the concentration range 0.06 – 0.82 μg/mL, LOD (3S) = 0.02 μg/mL, LOQ (10S) = 0.06 μg/mL. No interferences were observed in the presence of common components of the tablets such as microcrystalline cellulose, lactose monohydrate, corn starch, magnesium stearate, Opadry II 85F28751 White, polyethylene glycol 6000, polyvinyl alcohol, talc, titanium dioxide (E 171), citric acid, sodium bicarbonate, lemon flavor, adipic acid, povidone and aspartame (E 951). RSD = 1.45 % (δ = – 1.05 %), RSD = 0.64 % (δ = – 0.50 %) and RSD = 1.13 % (δ = – 0.16 %) for the ‟Acetylcystein 200 Heumann Brausetabletten” (Germany), ‟AC-FS” (‟Farmastart”, Ukraine) and ‟Acestad” tablets (Germany), respectively. The proposed method is promising for further research on the subject of its application for the determination of N-acetylcysteine in drugs, in the absence of ascorbic acid.

Keywords: N-acetylcysteine, luminol, inhibitor, chemilumeniscence method

Article language: En

Publisher:Taras Shevchenko National University, Kiev Ukraine

(Analytical chemistry department at Taras Shevchenko National University, 64 Vladimirskaya STR., Kiev, 01601 UKRAINE),, +380-44-2393345

Papers published in Methods and Objects of Chemical Analysis are considered as publications suitable for PhD thesis

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