Analytical chemistry council

METHODS AND OBJECTS OF CHEMICAL ANALYSIS

ISSN title: "Metody i obʺekty himičeskogo analiza"

An international journal devoted to all aspects of Analytical chemistry

ISSN 2413-6166 (Online), ISSN 1991-0290 (Print)

Kiev University
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HPLC separation of bioactive components of anti-inflammatory syrup on stationary phases with embedded polar groups

O.A. Syrotchuk, I.P. Didukh, S.F. Kuras, V.N. Zaitsev*

* Corresponding authors
SE «Central laboratory for quality control of medicines and medical products» Kudriavska Str., 10 G, 04053
Taras Shevchenko National University of Kyiv, Volodymyrska Str., 64, Kyiv, 01033
E-mail: zaitsev@univ.kiev.ua

Methods Objects Chem. Anal., 2015, 10(4), p. 171-177

https://doi.org/10.17721/moca.2015.171-177

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Abstract

For better separation of water-soluble biologically-active compounds by RP-HPLC in mobile phases with high content of water, new C18-type stationary phases with embedded polar groups (EPG-C18) were studied and the results were compared with those obtained on conventional C18 phases. Typical active ingredients of anti-inflammatory syrup, such as: paracetamol, phenylpropanolamine, caffeine and chlorpheniramine maleat were selected for investigation because of difficulties of their separation and also wide range of the polarity. It is demonstrated that complete HPLC separation of the components of the syrup cannot be achieved on any from studied C18 phases in 0.025M NaH2PO4 (H2O/CH3CN=9/1) mobile phase with pH interval 2.5-7.0. Contrary complete separation of the components can be easily achieved on EPG-С18 column. Because of essential changing of the retention time of the components with pH it has been assumed that separation of the compounds on EPG-С18 column includes both dispersion and dipole-dipole interactions of the embedded groups with polar group of the analyts.

Keywords: paracetamol, caffeine, phenylpropanolamine, chlorpheniramine, HPLC

Article language: Ukr

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Publisher:Taras Shevchenko National University, Kiev Ukraine

(Analytical chemistry department at Taras Shevchenko National University, 64 Vladimirskaya STR., Kiev, 01601 UKRAINE)
analysis@univ.kiev.ua, www.achem.univ.kiev.ua, +380-44-2393266

The journal is indexed in SCOPUS and Web of Science

The journal website: www.moca.net.ua